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Primer sequences used for quantitative real-time PCR

Journal: BMC Neuroscience

Article Title: Microglia-associated Galectin-3 expression in prenatal stress-induced cognitive dysfunction in adult offspring

doi: 10.1186/s12868-026-01048-9

Figure Lengend Snippet: Primer sequences used for quantitative real-time PCR

Article Snippet: Rat corticosterone (CORT) ELISA kit (Jianglai Biotech, JL12455); rat corticotropin-releasing hormone (CRH) ELISA kit (Jianglai Biotech, JL12541); Rat IL‐6, TNF‐α and IL‐1β ELISA kits were purchased from Wuhan Fine Biotech Co., Ltd. (Cat. No. ER0042, ER1094, ER1393); Anti-Galectin-3 antibody (Proteintech, 14979-1-AP); Rabbit polyclonal anti-NF-κB p65 (RELA) antibody (Proteintech, 10745-1-AP); Anti-Iba1 antibody (Abcam, ab178846); Anti-CD68 antibody (HUABIO, HA722285); Anti-GFAP antibody (Abcam, ab7260); Anti-NeuN antibody (HUABIO, ET1602-12); Anti-GAPDH antibody (Abcam, ab181602); Goat anti-rabbit IgG-HRP (ABclonal, AS014); Dual-label three-color multiplex immunofluorescence staining kit (enhanced version) (Aifang Biotech, AFIHC033); BCA protein quantification kit (Thermo Scientific); High-performance RIPA lysis buffer (Yaye Biotech, PC101); Protease inhibitor (Wuhan BoShide Bioengineering Co., Ltd., AR1179); One-step PAGE gel preparation kit (Yaye Biotech, PG222); High-speed low-temperature centrifuge (Hunan Xiangyi); Western blot electrophoresis instrument (Seville Bio); Animal behavior testing instruments and analysis software (Shanghai Jiliang Software Technology Co., Ltd.).

Techniques: Real-time Polymerase Chain Reaction